Journal: Cell Death & Disease

Article Title: Characterization of novel markers of senescence and their prognostic potential in cancer

doi: 10.1038/cddis.2014.489

Figure Lengend Snippet: Expression and localization of senescence markers. Immunofluorescent images of selected targets in EJp16 and EJp21 uninduced (Control) or 4 days after tet removal (Senescent), as well as early passage IMR90 human fibroblasts compared with those entering replicative senescence after serial passaging. Nucleus are stained with DAPI (blue)

Article Snippet: IMR90 (human fibroblasts derived from lungs of a 16-week female foetus) and normal human diploid fibroblasts (Cellworks, San Jose, CA, USA) were maintained in DMEM supplemented with 10% FBS, and penicillin–streptomycin (50 unit/ml) until they reached replicative senescence.

Techniques: Expressing, Control, Passaging, Staining

Journal: Cell Death & Disease

Article Title: Characterization of novel markers of senescence and their prognostic potential in cancer

doi: 10.1038/cddis.2014.489

Figure Lengend Snippet: Defining a new FACS-based protocol for the detection of senescent cells. ( a ) Representative plot analysis of fluorescence levels in control and senescent EJp16, HT1080p21-9 and human diploid fibroblasts (HDF) stained with fluorescently tagged antibodies against B2MG, DEP1 and NOTCH3, as measured by flow cytometry. Senescent cells were analysed after 5 days of p16 or p21 expression. Numbers indicate mean fluorescent intensity (MFI) values. ( b ) Average fold increases of MFI of the same cells when senescence is induced. Experiments were performed in triplicate. Error bars show S.D.

Article Snippet: IMR90 (human fibroblasts derived from lungs of a 16-week female foetus) and normal human diploid fibroblasts (Cellworks, San Jose, CA, USA) were maintained in DMEM supplemented with 10% FBS, and penicillin–streptomycin (50 unit/ml) until they reached replicative senescence.

Techniques: Fluorescence, Control, Staining, Flow Cytometry, Expressing